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Amiloride‐dependent transport is the main mechanism implicated in sodium iinflux regulation in rat mast cells
Author(s) -
Cabado Ana G.,
Vieytes Mercedes R.,
Botana Luis M.
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041560316
Subject(s) - amiloride , ouabain , tonicity , sodium , chemistry , mast cell , endocrinology , biophysics , medicine , isotonic , microbiology and biotechnology , pharmacology , biology , biochemistry , immunology , organic chemistry
Mast cell sodium regulation is a largely unknown field. In our effort to study the mechanisms by which mast cells regulate sodium levels, we have examined the effect of amiloride and ouabain on 22 Na entry in rat mast cells in isotonic and hypertonic conditions. Ouabain (0.5 mM) enhances sodium uptake by 32% in isotonic conditions. Hypertonicity increases by 400% the uptake of sodium through an amiloride (1 mM) dependent mechanism. Ouabain has no appreciable effect on the entry of 22 Na in hypertonic conditions. © 1993 Wiley‐Liss, Inc.