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Intracellular calcium oscillations induced in a T‐cell line by a weak 50 Hz magnetic field
Author(s) -
Lindströum Ewa,
Lindströum Per,
Berglund André,
Mild Kiell Hansson,
Lundgren Erik
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041560223
Subject(s) - jurkat cells , intracellular , calcium , calcium in biology , physics , magnetic field , biophysics , cell culture , chemistry , nuclear magnetic resonance , biology , microbiology and biotechnology , t cell , immunology , immune system , genetics , organic chemistry , quantum mechanics
Applied weak magnetic fields have been shown to affect cellular activity on several levels, but the mechanisms involved remain elusive. We have decided to study an early signal transduction event in the human T cell line Jurkat; oscillations of free [Ca 2+ ]i, of the type seen by crosslinking the CD3 complex. Cells were exposed to a 50 Hz, 0.1 mT, sinusoidal magnetic field while intracellular free calcium was measured in individual cells, using fura‐2 as a probe. An acute response was observed with oscillatory increases in [Ca 2+ ]i, which subsided when the field was turned off. The effect of the magnetic field on [Ca 2+ ]i was comparable to that achieved by an anti‐CD3 monoclonal antibody. © 1993 Wiley‐Liss, Inc.