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1,25‐dihydroxy vitamin D3 and 12‐O‐tetradecanoyl phorbol‐13‐acetate synergistically induce monocytic cell differentiation: FOS and RB expression
Author(s) -
Yen Andrew,
Coles Mark,
Varvayanis Susi
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041560126
Subject(s) - u937 cell , cellular differentiation , microbiology and biotechnology , chemistry , phorbol , tetradecanoylphorbol acetate , vitamin , cell culture , monocyte , biochemistry , biology , signal transduction , in vitro , immunology , protein kinase c , gene , genetics
1,25‐dihydroxy vitamin D3 and 12‐O‐tetradecanoyl phorbol‐13‐acetate (TPA) interact synergistically to induce monocytic differentiation of U937 histiocytic lymphoma cells. Addition of TPA causes an otherwise ineffective dose of 1,25‐dihydroxy vitamin D3 to induce differentiation. The induced differentiation depends on the simultaneous (vs. sequential) presence of both agents. The kinetics of induced differentiation are consistent with a G1 specific cellular response to initiate the metabolic cascade culminating in cell differentiation. The induced differentiation occurs with down‐regulation of c‐fos protein and an accompanying up‐regulation of RB protein expression, consistent with a possible need for up‐regulated RB expression to maintain a given differentiated phenotype and suppress transcriptional activators that might typically be associated with proliferation. © 1993 Wiley‐Liss, Inc.