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Decrease in cellular replicative potential in “giant” mice transfected with the bovine growth hormone gene correlates to shortened life span
Author(s) -
Pendergrass William R.,
Li Yi,
Jiang DeZhao,
Wolf Norman S.
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041560114
Subject(s) - biology , transfection , connective tissue , transgene , spleen , microbiology and biotechnology , bovine somatotropin , clone (java method) , in vitro , myofibroblast , cell culture , endocrinology , gene , medicine , immunology , hormone , growth hormone , fibrosis , genetics
Abstract Adult mice, (C57BL/6 × Sjl)F1 hybrids, transfected with the bovine growth hormone gene (bGH) grow to twice normal size, but have a mean life span less than 50% that of control siblings without the transgene. The replicative potentials of cells from six different tissue sites (tail skin and ear skin dermal fibroblasts, tail subdermal connective tissue fibroblasts, kidney medulla epithelial cells, bone marrow myofibroblasts, and spleen myofibroblasts) were assayed in vitro using clone size distribution analysis. Cells from all of the above bGH+ tissues produced a smaller fraction of large clones, relative to age‐matched controls, in all of these cell types. The loss of replicative potential did not appear to be the result of negative conditioning of the cloning media by the bGH+ cells, and was tightly correlated to the period of accelerated growth in these animals (3–12 weeks), a time when additional GH receptors are expressed. © 1993 Wiley‐Liss, Inc.