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Expression and loss of the transferrin receptor in growing and differentiating HD3 cells
Author(s) -
Grdisa Mira,
Mathew Anu,
Johnstone R. M.
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041550216
Subject(s) - erythroblast , exosome , transferrin receptor , microbiology and biotechnology , transferrin , biology , microvesicles , transporter , receptor , cell culture , nucleoside , chemistry , biochemistry , microrna , gene , genetics , haematopoiesis , stem cell
During induced differentiation and maturation of HD3 cells (a chicken erythroblast cell line infected with a temperature‐sensitive mutant of the avian erythroblastosis virus), the levels of transferrin receptor (TFR) and nucleoside transporter increase. Both these activities increase before elevated levels of hemoglobin are detected. Shortly after induction, as cellular TFR levels rise, a native‐size TFR is detected in the cell‐free culture medium, associated with an exosome fraction (100,000 xg pellet). Nucleoside transporter (measured as NBMPR‐binding activity) is not increased in this pellet with induction. Previous studies have suggested that exosome formation in peripheral reticulocytes may be a significant route for loss of specific membrane proteins (Johnstone et al., 1991). Although the present experiments in HD3 cells do not address the quantitative importance of exosome formation, the studies suggest that exosome formation is an early event in commitment to the red cell lineage and is not a phenomenon restricted to the terminal stages of red cell maturation. © 1993 Wiley‐Liss, Inc.