Premium
Position‐dependent nuclear accumulation of the retinoblastoma (RB) protein during in vitro myogenesis
Author(s) -
Szekely Laszlo,
Jin Pei,
Jiang WeiQin,
Rosén Anders,
Wiman Klas G.,
Klein George,
Ringertz Nils
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041550212
Subject(s) - myogenesis , multinucleate , biology , retinoblastoma protein , syncytium , microbiology and biotechnology , population , in vitro , myocyte , retinoblastoma , cell , cell cycle , virology , virus , biochemistry , demography , sociology , gene
The expression of the retinoblastoma (RB) protein has been studied during in vitro muscle differentiation by immunofluorescence staining with three different antibodies against RB protein. Proliferating mononucleate L6 rat myoblasts showed a low level of expression. As cells began to enter a nonreplicating G0 state, the cell population became heterogeneous. Some nonreplicating cells showed a high level of expression. Nuclei at the two ends of myotubes were strongly positive, whereas centrally located nuclei showed low RB expression. Overexpression of the human RB protein in rat L6 myotubes from a Semliki forest virus (SFV)‐based, transient expression vector produced a similar picture. Terminally located nuclei expressed human RB at a much higher level than did the centrally located nuclei. The results suggest that individual nuclei with a multinucleated syncytium may undergo position‐dependent specialization. © 1993 Wiley‐Liss, Inc.