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Serum‐stimulated cell cycle progression and stress protein synthesis in C3H10T1/2 fibroblasts treated with sodium arsenite
Author(s) -
Van Wijk Roeland,
Welters Marij,
Souren Jan E. M.,
Ovelgonne Hans,
Wiegant Fred A. C.
Publication year - 1993
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041550207
Subject(s) - sodium arsenite , arsenite , messenger rna , protein biosynthesis , cell growth , cell , heat shock protein , chemistry , downregulation and upregulation , biology , microbiology and biotechnology , biochemistry , gene , arsenic , organic chemistry
In this work, we demonstrate that a nonlethal dose of arsenite administered to quiescent C3H10T1/2 fibroblasts can enhance the mitogenic effect of suboptimal concentrations of serum. The mitogenic effect was dependent on the serum concentration and on the time interval between the administration of arsenite and that of serum. This suggests that mitogen sensitivity changes in time after arsenite treatment. It is shown that the concentrations of arsenite that enhance the mitogenic effect of serum also increase the mRNA levels of c‐fos, HSP68, and HSP84 and induce the specific synthesis of Heat Shock Proteins (HSPs). The physiological significance of this phenomenon is most likely to counteract the long‐term toxic effect of arsenite by early induction of compensation for cell loss. © 1993 Wiley‐Liss, Inc.