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Apoptosis of murine BW 5147 thymoma cells induced by cold shock
Author(s) -
Kruman Inna I.,
Gukovskaya Anna S.,
Petrunyaka Victor V.,
Beletsky Igor P.,
Trepakova Elena S.
Publication year - 1992
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041530115
Subject(s) - apoptosis , programmed cell death , cycloheximide , microbiology and biotechnology , biology , dna , cleavage (geology) , cytochalasin b , apoptotic dna fragmentation , dna fragmentation , fragmentation (computing) , chemistry , cell , biochemistry , protein biosynthesis , paleontology , ecology , fracture (geology)
Exposure of thymoma BW 5147 cells to cold (0–2°C) followed by rewarming at 37°C (cold shock) resulted in internucleosomal DNA cleavage. Sensitivity to cold shock‐induced cell death was critically dependent on the serum concentrations in the medium and limited to serum‐deficient medium (2% serum concentration), whereas cells in the complete growth medium (10%) were completely resistant. RNA/protein‐synthesis inhibitors (cycloheximide and actinomicin D) had no effect on cold shock‐induced DNA cleavage in BW 5147 cells. The DNA fragmentation seems to be independent of increase in the cytosolic Ca 2+ level. Moreover, reduction in the calcium content of the external medium by EGTA induced DNA cleavage. Incubation of BW 5147 cells in the presence of colchicine and cytochalasin B led to the apoptosis. The latter suggest that the internucleosomal DNA cleavage induced by cold shock may be concerned with the disruption of some cytoskeletal network caused by cooling. The results are discussed in relation to cell proliferation. © 1992 Wiley‐Liss, Inc.