Premium
Independent regulation of prostaglandin production and the stress response in human fibroblasts
Author(s) -
Salzman J.,
Bowman P. D.
Publication year - 1992
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041520322
Subject(s) - sodium arsenite , prostaglandin , prostaglandin e2 , cycloheximide , chemistry , cyclooxygenase , arsenite , protein biosynthesis , biochemistry , prostaglandin e , endocrinology , medicine , enzyme , biology , arsenic , organic chemistry
The stress, or heat shock response of eukaryotic cells is characterized by dramatic changes in the metabolism of responding cells, most notably the increased synthesis of a group of proteins known as heat shock proteins. In this study, we examined the relationship of prostaglandin synthesis/release to the stress response. Stress protein synthesis was induced with sodium arsenite, and prostaglandin E 2 and prostacyclin (measured as 6‐keto PGF 1α ) levels were determined by enzyme immunoassay. The stress response was monitored by the incorporation of [ 35 S]methionine and separation of protein by one‐dimensional sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Prostaglandin synthesis and the stress response were both induced by sodium arsenite. However, aspirin, a cyclooxygenase inhibitor, inhibited arsenite‐induced prostaglandin synthesis but did not inhibit stress protein synthesis. Conversely, the calcium ionophore A23187 also stimulated prostaglandin synthesis, but did not induce the stress response. The results of this study indicate that sodium arsenite, a stress response inducer, stimulates prostaglandin production, but this appears to be a correlative rather than causative occurrence in the stress response. Determination of the cytotoxicity of arsenite indicated a high correlation of stimulation of prostaglandin release with cytotoxicity. © 1992 Wiley‐Liss, Inc.