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Signal transduction during liver regeneration: Role of insulin and vasopressin
Author(s) -
Marino M.,
Mangiantini M. T.,
Spagnuolo S.,
Luly P.,
Leoni S.
Publication year - 1992
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041520223
Subject(s) - inositol , liver regeneration , phospholipase c , insulin , phospholipase , inositol phosphate , biology , second messenger system , endocrinology , medicine , vasopressin , biochemistry , liver cell , signal transduction , chemistry , microbiology and biotechnology , regeneration (biology) , receptor , enzyme
The relationship between cell proliferation and inositol lipid turnover has been studied by comparing the steady state of inositol derivative metabolism in quiescent and regenerating rat hepatocytes isolated at 4 h (G1 phase of first cell cycle) and 24 h (onset of M phase) after partial hepatectomy. The effect of two hormones able to regulate hepatic regeneration, insulin and vasopressin, has been considered, and the results can be summarized as follows: (i) at 4 h after partial hepatectomy, the precursor incorporation into inositol polyphosphates and the particulate phospholipase C activity increase with respect to quiescent hepatocytes, whereas the content of I1,4,5P 3 does not change, suggesting an increased turnover of this molecule in this step of cell cycle priming; (ii) 24 h after partial hepatectomy, the radioactivity linked to IP 3 and IP 4 , as well as soluble and particulate phospholipase C activity, and IP 3 content increase, suggesting the presence, at the onset of M phase, of second messenger accumulation; (iii) only 24 h after partial hepatectomy, the inositol derivative metabolism is affected by vasopressin; and (iv) insulin exerts a modulatory role on inositol polyphosphate production without involving membrane‐bound PLC activity or phosphoinositide hydrolysis. These data suggest that inositol‐derived signal molecules are associated with hepatic regneration; moreover, the metabolic pathway of such compounds seems to be regulated so that only specific inositol phosphates are present in each step of the cell cycle. © 1992 Wiley‐Liss, Inc.