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Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo
Author(s) -
Umezawa Akihiro,
Maruyama Tatsuya,
Segawa Kaoru,
Shadduck Richard K.,
Waheed Abdul,
Hata JunIchi
Publication year - 1992
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041510125
Subject(s) - haematopoiesis , in vivo , stromal cell , microbiology and biotechnology , bone marrow , cell culture , multipotent stem cell , chemistry , biology , stem cell , immunology , cancer research , progenitor cell , genetics
Abstract Several murine marrow stromal cells were established from murine bone marrow cultures. Stromal cell lines transfected with a tumor‐inducing polyoma virus middle T antigen (MTAg) were inoculated into nude mice subcutaneously. KUSA‐MTAg cells, one of these cell lines, led to the rapid local development of bone marrow consisting of trilineage hemantopoietic cells and bone; other cell lines produced spindle cell sarcoma or hemangiosarcoma. These results suggested that a single stromal cell line, KUSA‐MTAg cells, may induce hematopoietic stem cells or early progenitors of three lineages of hematopoietic cells in vivo. Interestingly, untransfected KUSA cells expressed three new mesenchymal phenotypes, osteocytes, adipocytes, and myotubes, after treatment with 5‐azacytidine. © 1992 Wiley‐Liss, Inc.