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Keratinocytes stimulate prostaglandin I 2 synthesis by 3T3 cells and exhibit enhanced cornification when exposed to prostaglandin I 2 analogues
Author(s) -
Baden Howard P.,
Goldaber Michael L.,
Kvedar Joseph C.
Publication year - 1992
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041500208
Subject(s) - 3t3 cells , keratinocyte , prostaglandin , involucrin , cyclooxygenase , prostaglandin e2 , cell culture , biology , endocrinology , microbiology and biotechnology , medicine , biochemistry , in vitro , enzyme , transfection , genetics
Abstract The predominant cyclooxygenase products of keratinocytes are prostaglandin (PG)E 2 and PGF 2α with only trace amounts of PGI 2 synthesis detected. When normal or immortal (NM1) keratinocytes were co‐cultured with mitomycin C‐treated 3T3 cells, increased synthesis of PGI 2 was noted compared to mitomycin C‐treated 3T3 cells alone. The PGI 2 level in co‐cultures was maximum within the first week and diminished rapidly thereafter. These results suggested keratinocytes enhance the production of PGI 2 by 3T3 cells. Keratinocyte cultures incubated with lloprost and Piriprost, stable PGI 2 analogues, showed evidence of increased cornification as demonstrated by staining with rhodanile blue, decreased shedding of cells into the culture medium, and more cornified material adhering to the culture surface. The cultures appeared to be responsive between the first and second weeks after plating and the inhibition of shedding could not be reversed by changing to drug‐free medium. Control and treated cultures showed identical electrophoretic protein patterns. Immunoblots showed involucrin unchanged in extracts of control and treated cultures while the 22 kd pancornulin was absent in treated cultures. The findings that keratinocytes enhance the production of PGI 2 by 3T3 cells and that PGI 2 analogues enhance cornification of confluent keratinocytes raise the possibility that eicosanoids may serve as autoregulatory signals together with other factors.