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Il‐1β and prostaglandins regulate integrin mRNA expression
Author(s) -
Milam Stephen B.,
Magnuson Victoria L.,
Steffensen Bjorn,
Chen Dali,
Klebe Robert J.
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041490202
Subject(s) - integrin , beta (programming language) , messenger rna , alpha (finance) , gene expression , protein subunit , biology , microbiology and biotechnology , endocrinology , medicine , cell , chemistry , gene , biochemistry , construct validity , nursing , computer science , patient satisfaction , programming language
The purpose of this study was to examine the effects of IL‐1β on integrin expression in MG‐63 human osteosarcoma cells. Human recombinant IL‐1β (rIL‐1β) produced significant increases in both α 2 ‐ and α 5 ‐subunit mRNA levels, as well as a smaller increase in α v ‐subunit mRNA. In contrast, IL‐1β decreased α 4 ‐subunit mRNA levels by approximately 30% relative to untreated controls. These findings suggest that human IL‐1β differentially regulates expression of integrins. When cultures were treated with both IL‐1β and the cyclooxygenase inhibitor, indomethacin, the expression of α 2 ‐, α 5 ‐, and α v ‐subunit mRNA levels were dramatically increased relative to untreated controls; co‐treatment with 0.5 mM prostaglandin E 2 (PGE 2 ) partially reversed this effect. Indomethacin alone did not affect integrin mRNA levels. Treatment with IL‐1β or IL‐1β + indomethacin also induced significant changes in MG‐63 morphology (i.e., increased cell elongation) and increased the ability of cells to contract collagen gels. PGE 2 reversed the above effects on cell morphology and gel contraction. These findings indicate that (a) IL‐1β differentially regulates the expression of integrins and (b) that PGE 2 , which is induced by IL‐1β, may provide a negative feedback loop which counteracts the stimulatory effect of IL‐1β on integrin gene expression. It is suggested that products of inflammation may affect cell behavior by differentially regulating the expression of various integrins.