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Human keratinocyte growth‐promoting activity on the surface of fibroblasts
Author(s) -
Yaeger Peter C.,
Stiles Charles D.,
Rollins Barrett J.
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041490114
Subject(s) - keratinocyte , microbiology and biotechnology , chemistry , cell growth , biology , biochemistry , in vitro
To proliferate in serum‐containing medium, normal human keratinocytes must be co‐cultured with fibroblast feeder cells. Conditioned medium from feeder cell cultures cannot substitute for the cells themselves. We tested the hypothesis that fibroblasts display a keratinocyte growth‐promoting activity on their outer cell surface. The results of our investigation showed that (1) glutaraldehyde‐fixed fibroblast feeder cells promote keratinocyte growth, (2) the growth‐promoting effect requires contact between fixed fibroblasts and keratinocytes, and (3) feeder activity is highly enriched within the plasma membrane fraction of fibroblasts. We conclude that at least part of the fibroblast “feeder” activity involves a keratinocyte growth‐promoting factor which is bound to the outer surface of fibroblast plasma membranes.