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Murine mast cell colony formation supported by IL‐3, IL‐4, and recombinant rat stem cell factor, ligand for c‐kit
Author(s) -
Tsuji Kohichiro,
Zsebo Krisztina M.,
Ogawa Makio
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041480306
Subject(s) - stem cell factor , mast cell , biology , cytokine , interleukin 3 , stem cell , growth factor , bone marrow , immunology , haematopoiesis , interleukin 5 , interleukin 4 , microbiology and biotechnology , interleukin , receptor , biochemistry , t cell , antigen presenting cell , immune system
Recently, a novel cytokine designated stem cell factor (SCF) was isolated from medium conditioned by buffalo rat liver cells and proved to be the ligand for c‐kit. We have examined the effects of recombinant rat SCF alone and in various combinations with interleukin‐3 and interleukin‐4 on murine mast cell colony formation in methylcellulose culture. As a source of connective tissue‐type mast cells (CTMC), we used peritoneal mast cells. No individual factor supported colony formation by purified peritoneal mast cells. When cells were grown in combinations of two factors, significant mast cell colony growth was seen. When cells were grown in the presence of three factors, not only the number of colonies was increased but also the colonies were larger. Mast cells in these colonies contained safranin‐ and berberine sulfate‐positive cells, but the proportions of positive and negative cells varied depending on the factor combinations. We then examined the effects of these factors on proliferation of bone marrow‐derived mast cells (BMMC) by replating pooled mast cell colonies. As a single factor, only interleukin‐3 supported mast cell colony formation. Combinations of two of the three factors supported mast cell colony formation. However, the most impressive synergism was seen again with the combination of the three factors. Not only was the number of colonies increased, but there was a significant increase in size. These results indicate that SCF is an important factor for the proliferation of both CTMC and BMMC.