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Establishment of a variety of human bone marrow stromal cell lines by the recombinant SV40‐adenovirus vector
Author(s) -
Aizawa Shin,
Yaguchi Makoto,
Nakano Masaru,
Inokuchi Sadaki,
Handa Hiroshi,
Toyama Keisuke
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041480209
Subject(s) - recombinant dna , stromal cell , cell culture , human bone , transfection , bone marrow , biology , vector (molecular biology) , viral vector , virology , cell , microbiology and biotechnology , in vitro , immunology , cancer research , gene , genetics
Various human bone marrow stromal cell lines were established from the adherent cell populations by introduction of the recombinant SV40‐adenovirus vector with an infection or electric poration procedure. As compared with DMA transfection, the vector introduction was able to immortalize the cells with more than 100 times higher efficiency. Morphological and cytochemical analyses indicated that various cloned cell lines with different properties were isolated by the vector introduction. All the established cell lines expressed SV40 large T antigen. These results provided the evidence indicating that the recombinant SV40‐adenovirus vector was a useful tool to establish a variety of cell lines with different biological activities from human bone marrow stroma.