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Bovine aortic endothelial cells release hydrogen peroxide
Author(s) -
Sundqvist T.
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041480118
Subject(s) - hydrogen peroxide , horseradish peroxidase , catalase , chemistry , superoxide dismutase , chemiluminescence , peroxidase , microcarrier , stimulation , superoxide , bradykinin , biochemistry , extracellular , enzyme , biophysics , biology , cell , endocrinology , chromatography , receptor
Endothelial cells grown on microcarriers are able to release H 2 O 2 to the extracellular environment without any added stimulus. The extracellularly released H 2 O 2 can be detected by luminol‐amplified chemiluminescence (CL) if horseradish peroxidase is added. The CL response can be reduced by catalase and blocked by superoxide dismutase, indicating that O 2 ‐could be a precursor for H 2 O 2 . The CL kinetics, i.e., a long lag time followed by a rapid shift to a new level, indicate activation of an O 2 ‐producing enzyme. The cells are also able to protect themselves from H 2 O 2 stimulation by both catalase and the glutatione system. Bradykinin stimulates the H 2 O 2 release, but if the effect is directly stimulatory or if it acts by reduction of the protective system is at present unclear. The extracellularly released H 2 O 2 could be a cause of injury to the endothelial cells or to the subendothelial matrix.