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Association of c‐myc protein with enzymes of DNA replication in high molecular weight fractions from mammalian cells
Author(s) -
Studzinski George P.,
Shankavaram Uma T.,
Moore Dorothy C.,
Reddy Prem Veer
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041470305
Subject(s) - dna replication , enzyme , microbiology and biotechnology , biology , dna , nuclear protein , transformation (genetics) , dna polymerase , proliferating cell nuclear antigen , function (biology) , biochemistry , dna polymerase delta , gene , polymerase chain reaction , transcription factor , reverse transcriptase
Abstract Oncoprotein c‐myc is expressed in proliferating but not quiescent mammalian cells, and its overexpression or inappropriate expression is associated with malignant transformation. However, in spite of an intense interest, the normal function of this protein has remained elusive. As a step towards the elucidation of the function of c‐myc protein, we studied its distribution within several types of cells, including HL 60, K 562, COLO 320, and CHEF/18 cells. In all of the cells studied, c‐myc protein was detected in high molecular weight protein fractions, in 350–600 Kd range, in gel‐exclusion chromatography and sucrose gradient centrifugation. This distribution of c‐myc protein coincided with the distribution of DNA polymerase α and several other enzymes necessary for DNA replication. The data suggest that c‐myc product may be a component of the replitase complex of enzymes involved in nuclear DNA replication.