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In vitro kinetics of basic fibroblast growth factor diffusion across a reconstituted corneal endothelium
Author(s) -
Dabin Isabelle,
Courtois Yves
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041470303
Subject(s) - matrigel , basic fibroblast growth factor , basement membrane , biophysics , in vitro , chemistry , endothelium , membrane , permeability (electromagnetism) , corneal endothelium , microbiology and biotechnology , growth factor , biology , biochemistry , endocrinology , receptor
We have studied the ability of bFGF to traverse and be trapped within basement membranes. An extract of EHS tumor (matrigel) coated on culture chamber filters was used as an in vitro model of basement membranes. Our results showed a slow diffusion of bPGF dependent on the amount of low affinity binding sites present within the matrigel. High amounts 01 bFGF and heparin increased the initial rate of diffusion by displacement of bFGF bound to matrigel. An in vitro corneal endothelium model (endothelial cells overlying matrigel) was also developed. This monolayer, with a weak permeability, decreased the kinetic rate of bFGF diffusion compared with matrigel alone. These results indicate that modulation of bFGF distribution in a tissue by a basement membrane is dependent on bFGF concentration, basement membrane composition, permeability of associated cells, and local presence of heparin. This selective control may be a regulating step in bFGF action.