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Colony stimulating factor‐1 stimulates diacylglycerol generation in murine bone marrow‐derived macrophages, but not in resident peritoneal macrophages
Author(s) -
Veis Nurin,
Hamilton John A.
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041470215
Subject(s) - diacylglycerol kinase , zymosan , lipopolysaccharide , tumor necrosis factor alpha , macrophage , biology , phosphatidylinositol , colony stimulating factor , bone marrow , endocrinology , microbiology and biotechnology , chemistry , medicine , immunology , protein kinase c , biochemistry , haematopoiesis , signal transduction , in vitro , stem cell
Colony stimulating factor‐1 (CSF‐1) stimulates DNA synthesis in murine bone marrow‐derived macrophages (BMM); however, unlike BMM, murine resident peritoneal macrophages (RPM) undergo a poor proliferative response. It has previously been shown that phosphatidylinositol‐4,5‐bisphosphate hydrolysis is not associated with CSF‐1 action in BMM. In this report we demonstrate that, despite a lack of inositol trisphosphate generation, CSF‐1 transiently elevated both [ 3 H]myristoyl‐ and [ 3 H]arachidonyl‐diacylglycerol (DAG) in BMM in a dose‐dependent fashion. CSF‐1 failed, however, to stimulate an increase in either species of DAG in RPM. Thus, DAG could be a second messenger for the proliferative action of CSF‐1 in macrophages. Other mitogenic agents, 12‐O‐tetradecanoyl phorbol 13‐acetate (TPA) and exogenous phospholipase C, also increased BMM levels of [ 3 H]myristoyl‐ and [ 3 H]arachidonyl‐DAG. The nonmitogenic agents, lipopolysaccharide (LPS), tumor necrosis factor‐α (TNF‐α) and zymosan, had different effects on the generation of either species of DAG in BMM. LPS failed to elevate either form, TNF‐α increased only [ 3 H]arachidonyl‐DAG, while zymosan stimulated levels of both species of DAG. It therefore appears that increased diacylglycerol generation may be necessary, but perhaps not sufficient, for macrophage proliferation.