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Effects of a sialoglycopeptide on early events associated with signal transduction
Author(s) -
TooleSimms W. E.,
Loder D. K.,
Fattaey H. K.,
Johnson T. C.
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041470214
Subject(s) - cytosol , signal transduction , microbiology and biotechnology , transduction (biophysics) , cell , 3t3 cells , biology , antagonist , receptor , calcium , second messenger system , cell growth , 12 o tetradecanoylphorbol 13 acetate , biochemistry , cell culture , chemistry , protein kinase c , enzyme , genetics , transfection , organic chemistry , phorbol ester
A sialoglycopeptide (SGP), isolated and purified from bovine cerebral cortex cells, was studied in regard to early signal transduction events associated with the cell cycle. Previously shown to be a potent antagonist to a variety of mitogens, the SGP abrogated the ability of 12‐O‐tetradecanoylphorbol‐13 acetate (TPA) to elicit an alkalinization of 3T3 cell cytosol, but only when added minutes prior to, or simultaneously with, the tumor promoter. 3T3 cell TPA‐mediated Ca 2+ mobilization was also inhibited by the SGP although the inhibitor itself did not bind Ca 2+ in a cell‐free assay. The results are discussed in light of the already known kinetics of interaction between the SGP, various mitogens, and the calcium ionophore A23187 with regard to the pivotal events leading to the decision of a cell to divide or not to divide.