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Developmental patterns in the antioxidant defenses of the housefly, Musca domestica
Author(s) -
Allen R. G.,
Oberley L. W.,
Elwell J. H.,
Sohal R. S.
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041460212
Subject(s) - housefly , glutathione , glutathione reductase , biochemistry , musca , catalase , superoxide dismutase , glutathione peroxidase , biology , enzyme , antioxidant , oxidizing agent , chemistry , botany , organic chemistry , larva
The activities of superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione S‐transferases, GSSG reductase, thiol transferases, gamma glutamyl‐cysteine synthetase, and glucose‐6‐phosphate dehydrogenase, and the concentrations of H 2 O 2 and reduced and oxidized glutathione were determined in the various developmental stages of houseflies. Housefly development was correlated with a progressive increase of cellular oxidizing equivalents and a loss of cellular reducing capacity. The loss of reducing equivalents appeared to result from a decrease in the activity of enzymes involved in glutathione and NADPH synthesis and a concomitant increase in glutathione‐oxidizing enzymes. Relatively little change was observed in SOD activity during housefly development; however, the electrophoretic pattern of MnSOD varied in a manner specific to developmental stage. A striking increase in H 2 O 2 concentration occurred prior to pupation possibly due to changes in substrate catabolism. These results support the hypothesis that the cellular environment becomes progressively more oxidizing during development.