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IL‐3 specifically inhibits GM‐CSF binding to the higher affinity receptor
Author(s) -
Taketazu Fumitoshi,
Chiba Shigeru,
Shibuya Kyoichi,
Kuwaki Tomoaki,
Tsumura Haruhiko,
Miyazono Kohei,
Miyagawa Kiyoshi,
Takaku Fumimaro
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041460209
Subject(s) - receptor , microbiology and biotechnology , cell culture , granulocyte macrophage colony stimulating factor , biology , cell surface receptor , haematopoiesis , chemistry , cytokine , biochemistry , immunology , stem cell , genetics
The inhibition of binding between human granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) and its receptor by human interleukin‐3 (IL‐3) was observed in myelogenous leukemia cell line KG‐1 which bore the receptors both for GM‐CSF and IL‐3. In contrast, this phenomenon was not observed in histiocytic lymphoma cell line U‐937 or in gastric carcinoma cell line KATO III, both of which have apparent GM‐CSF receptor but an undetectable IL‐3 receptor. In KG‐1 cells, the cross‐inhibition was preferentially observed when the binding of GM‐CSF was performed under the high‐affinity binding condition; i.e., a low concentration of 125 I‐GM‐CSF was incubated. Scatchard analysis of 125 I‐GM‐CSF binding to KG‐1 cells in the absence and in the presence of unlabeled IL‐3 demonstrated that IL‐3 inhibited GM‐CSF binding to the higher‐affinity component of GM‐CSF receptor on KG‐1 cells. Moreover, a chemical cross‐linking study has revealed that the cross‐inhibition of the GM‐CSF binding observed in KG‐1 cells is specific for the β‐chain, M r 135,000 binding protein which has been identified as a component forming the high‐affinity GM‐CSF receptor existng specifically on hemopoietic cells.