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Distribution and comparison of receptors for leukemia inhibitory factor on murine hemopoietic and hepatic cells
Author(s) -
Hilton Douglas J.,
Nicola Nicos A.,
Metcalf Donald
Publication year - 1991
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041460204
Subject(s) - haematopoiesis , receptor , inhibitory postsynaptic potential , distribution (mathematics) , leukemia , leukemia inhibitory factor receptor , biology , leukemia inhibitory factor , immunology , microbiology and biotechnology , cancer research , neuroscience , mathematics , genetics , stem cell , cytokine , interleukin 6 , mathematical analysis
Leukemia inhibitory factor (LIF) is a glycoprotein that induces the differentiation of the monocytic leukemia cell line M1 but suppresses the differentiation of totipotent embryonic stem cells. In an attempt to define the normal cellular targets for LIF, the distribution of LIF receptors within hemopoietic and hepatic tissue was analyzed by binding cells with radioiodinated LIF ( 125 I‐LIF) and subsequently carrying out autoradiography. Autoradiography demonstrated that in each he‐mopoietic tissue examined cells of monocyte/macrophage lineage were the primary cell type labeled with 125 I‐LIF. Moreover, both fetal and adult parenchy‐mal hepatocytes displayed higher levels of labeling than either monocytes or macrophages. The number of receptors per positive cell varied from 150 for bone marrow monocytes to 2,000 for adult hepatocytes. In each case, however, binding was of high affinity, with an apparent K D of 34–100 pM, and binding was specific, since labeling was competed for by unlabeled LIF but not a range of other structurally unrelated growth and differentiation factors. It is suggested that LIF may play a role in regulating macrophage function and hepatic acute phase protein synthesis in response to infection.