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Differential modes of action of fibronectin and epidermal growth factor on rabbit corneal epithelial migration
Author(s) -
Nishida Teruo,
Nakamura Masatsugu,
Mishima Hiroshi,
Otori Toshifumi
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041450323
Subject(s) - epidermal growth factor , fibronectin , epithelium , corneal epithelium , cornea , stimulation , microbiology and biotechnology , cell migration , chemistry , endocrinology , biology , medicine , receptor , cell , extracellular matrix , biochemistry , neuroscience , genetics
In order to clarify the roles of fibronectin (FN) and epidermal growth factor (EGF) in corneal wound healing, we cultured blocks of excised rabbit cornea for 24 hours in media containing one of these agents, then measured the length of the path of the epithelial layer that had migrated down the side of the block. Both FN and EGF stimulated epithelial migration significantly in a dose‐dependent fashion. Responses to EGF involved a time ag of at least 12 hours before stimulation could be observed, but there was no lag‐time for FN‐stimulated migration. FN was maximally effective only if it was continuously present. In contrast, exposure to EGF for 6 hours did not stimulate epithelial migration, but exposure for 9 hours resulted in the same stimulatory effects as were observed after 24 hours' continuous exposure. Anti‐FN antibody inhibited the FN‐ and EGF‐stimulated migration of corneal epithelium. But anti‐EGF antibody inhibited only EGF‐stimulated migration and had no effect on FN‐stimulated migration. These results indicate that, unlike FN, EGF need not be present, once the epithelial cells have recognized its signal. Furthermore, the stimulatory effect of EGF depended on FN, while that of FN was independent of EGF. The effects of EGF on migration of corneal epithelium may, therefore, be mediated by FN.