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Growth factor‐induced DNA synthesis in cells that overproduce protein kinase C
Author(s) -
Hoshina Sadayori,
Ueffing Marius,
Weinstein I. Bernard
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041450210
Subject(s) - protein kinase c , dna synthesis , cell culture , biology , cell growth , growth factor , microbiology and biotechnology , cell , phenotype , platelet derived growth factor receptor , in vitro , kinase , biochemistry , genetics , gene , receptor
Abstract In previous studies (Housey et al.: Cell 52: 343–354, 1988), our laboratory demonstrated that a cell line R6‐PKC3 that stably overproduces high levels of the βisoform of PKC displayed several abnormalities in growth control, and these phenotypic changes were also markedly enhanced when the cells were exposed to TPA. The present studies indicate that these cells also display marked changes in their response to certain growth factors. A striking finding was that several agents when tested alone in serum‐free medium, including EGF, PDGF, TPA, teleocidin, and OAG, stimulated DNA synthesis in quiescent R6‐PKC3 cells but had a negligible effect in quiescent R6‐C1 cells, a vector control cell line with jiormal levels of PKC. R6‐PKC3 cells also show an exaggerated response to very low concentrations of serum, when compared to R6‐C1 control cells. These studies provide direct genetic evidence that alterations in ce lular levels of PKC can markedly influence the responses of cells to specific growth factors.