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Regulation of tubulin synthesis during the cell cycle in the synchronous plasmodia of Physarum polycephalum
Author(s) -
Ducommun B.,
Cance J.,
Wright M.
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041450117
Subject(s) - tubulin , physarum polycephalum , aphidicolin , dna synthesis , mitosis , microbiology and biotechnology , biology , microtubule , cell cycle , isotype , physarum , biochemistry , cell , dna , genetics , antibody , monoclonal antibody
Regulation of α‐ and β‐tubulin isotype synthesis during the cell cycle has been studied in the myxomycete Physarum polycephalum , by subjecting synchronous plasmodia to temperature shifts and pharmacological perturbations. Temperature *shifts interfered with the regulation of tubulin synthesis. Inhibition of DNA synthesis prevents tubulin degradation after completion of the cell cycle (Ducommun and Wright, Eur. J. Cell Biol., 50:48–55, 1989) but did not perturb the initiation of tubulin synthesis. The constant increase of tubulin synthesis in the presence of tubulin‐sequestering drugs and the decrease of tubulin synthesis during a treatment with aphidicolin in late G2 phase suggest the existence of an autoregulatory mechanism of tubulin synthesis. Moreover, the microtubule poison methyl benzimidazole carbamate dissociated synthesis of the α 1 ‐tubulin isotype from the generally strictly coordinated synthesis of all tubulin isotypes during the transient interruption of mitosis. These observations show that a microtubular poison can perturb regulation of the synthesis of specific isotubulins.