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Bumetanide‐sensitive Na+ /K+ /Cl− transporter is stimulated by phorbol ester and different mitogens in quiescent human skin fibroblasts
Author(s) -
Panet Rivka,
Atlan Henri
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041450106
Subject(s) - protein kinase c , bumetanide , growth factor , phorbol , fibroblast growth factor , chemistry , platelet derived growth factor receptor , platelet derived growth factor , fibroblast , epidermal growth factor , endocrinology , medicine , protein kinase a , microbiology and biotechnology , signal transduction , biology , biochemistry , kinase , receptor , in vitro , ion transporter , membrane
In this study we investigated the correlation between the mitogenic effect and stimulation of Rb + (K + ) fluxes in human skin fibroblasts treated by purified growth factors. Both K+ transporters, bumetanide‐sensitive and ouabain‐sensitive, are stimulated 2‐3‐fold after addition of either fetal calf serum or purified recombinant growth factors to quiescent G 0 /G 1 human skin fibroblasts. Three groups of mitogens were compared: (i) the phorbol ester 2‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA); (ii) growth factors that stimulate inositol phosphate hydrolysis and subsequently activate protein kinase C—fibroblast growth factor (FGF), platelet derived growth factor (PDGF), and α‐thrombin; and (iii) growth factors that do not activate kinase C—insulin‐like growth factor‐1 (IGF‐1), and transforming like growth‐factor‐α (TGF‐α). The three groups of mitogens stimulated human skin fibroblasts proliferation and Rb+ influxes in a similar dose‐dependent fashion. The results indicate that both the bumetanide‐sensitive and the ouabain‐sensitive Rb+ fluxes are stimulated by protein kinase C‐dependent and by the protein kinase C‐independent pathways of the mitogenic signal.