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Nuclear and cytoplasmic localization of different basic fibroblast growth factor species
Author(s) -
Renko M.,
Quarto N.,
Morimoto T.,
Rifkin D. B.
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041440114
Subject(s) - basic fibroblast growth factor , cell fractionation , cytoplasm , subcellular localization , immunofluorescence , microbiology and biotechnology , biology , cytosol , growth factor , nuclear localization sequence , fibroblast growth factor , immunocytochemistry , transfection , complementary dna , antibody , biochemistry , gene , immunology , endocrinology , enzyme , receptor
The subcellular distribution of basic fibroblastic growth factor (bFGF) was analyzed by subcellular fractionation and immunofluorescence to gain insight into potential mechanisms for its release from cells. Subcellular fractionation of either SK‐Hep‐1 cells or NIH 3T3 cells transfected with a bFGF cDNA revealed that the 18 kd form of bFGF was found primarily in the cytosolic fraction, whereas the 22 and 24 kd forms of bFGF were found preferentially in ribosomal and nuclear fractions. Analysis of bFGF distribution by immunofluorescence using an antibody that recognized all forms of bFGF indicated both cytoplasmic and nuclear localization but failed to reveal any growth factor in structures representing secretory vesicles. Therefore, bFGF has a distribution inconsistent with that of a secretory protein.