Participation of transient‐type Ca 2+  channels in the sustained increase of Ca 2+  level in GH 3  cells | Zendy

Suzuki Nobuyuki | Zendy; Kano Masaakira | Zendy; Tanakadate Akihiro | Zendy; Kudo Yoshihisa | Zendy; Takagi Hiroshi | Zendy; Yoshioka Tohru | Zendy

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Participation of transient‐type Ca 2+ channels in the sustained increase of Ca 2+ level in GH 3 cells

Author(s) -

Suzuki Nobuyuki,

Kano Masaakira,

Tanakadate Akihiro,

Kudo Yoshihisa,

Takagi Hiroshi,

Yoshioka Tohru

Publication year - 1990

Publication title -

journal of cellular physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.529

H-Index - 174

eISSN - 1097-4652

pISSN - 0021-9541

DOI - 10.1002/jcp.1041440109

Subject(s) - chemistry , endocrinology , medicine , channel blocker , biophysics , thyrotropin releasing hormone , cytosol , voltage dependent calcium channel , patch clamp , calcium , fura 2 , hormone , biochemistry , biology , receptor , enzyme

Participation of two types of Ca 2+ channels (T‐ and L‐types) in the sustained increase of cytosolic‐free Ca 2+ concentration ([Ca 2+ ] i ) was studied in thyrotropin‐releasing hormone (TRH)‐stimulated clonal GH 3 pituitary cells. The effects of Ca 2+ channel blockers were analyzed by measuring Ca 2+ channel current and [Ca 2+ ] i , using whole‐cell voltage‐clamp and Fura‐2 fluorometry, respectively. Phenytoin (100 μM) and Ni 2+ (100 μM) selectively blocked T‐type Ca 2+ channels and suppressed the TRH‐induced sustained [Ca 2+ ] i increase in single cells. Synthetic ω‐conotoxin (ω‐CgTX, 2 μM) preferentially blocked L‐type Ca 2+ channels, but it did not suppress the TRH‐induced sustained [Ca 2+ ] i increase. The present results suggest that the sustained elevations of [Ca 2+ ] i triggered by TRH may be mediated by T‐type Ca 2+ channels in GH 3 cells.

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