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Studies on the energy metabolism of opossum ( Didelphis virginiana ) erythrocytes: V. Utilization of hypoxanthine for the synthesis of adenine and guanine nucleotides in vitro
Author(s) -
Bethlenfalvay N. C.,
White J. C.,
Chadwick E.,
Lima J. E.
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041430322
Subject(s) - hypoxanthine , guanine , nucleotide , didelphis , gtp' , biochemistry , purine , chemistry , opossum , hemoglobin , in vitro , biology , enzyme , anatomy , gene
High pressure liquid radiochromatography was used to test the ability of opossum erythrocytes to incorporate tracer amounts of [G‐ 3 H] hypoxanthine (Hy) into [ 3 H] labelled triphosphates of adenine and guanine. In the presence of supraphysiologic (30 mM) phosphate which is optimal for PRPP synthesis, both ATP and GTP are extensively labelled. When physiologic (1 mM) medium phosphate is used, red cells incubated under an atmosphere of nitrogen accumulate [ 3 H] ATP in a linear fashion suggesting ongoing PRPP synthesis in red cells whose hemoglobin is deoxygenated. In contrast, a lesser increase of labelled ATP is observed in cells incubated under oxygen, suggesting that conditions for purine nucleotide formation from ambient Hy are more favorable in the venous circulation.