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Acidic fibroblast growth factor (HBGF‐1) stimulates DNA synthesis in primary rat hepatocyte cultures
Author(s) -
Houck Keith A.,
Zarnegar Reza,
Muga Stephanie J.,
Michalopoulos George K.
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041430117
Subject(s) - hepatocyte , dna synthesis , fibroblast growth factor , epidermal growth factor , hepatocyte growth factor , growth factor , fibroblast , biology , microbiology and biotechnology , endocrinology , medicine , dna , chemistry , cell culture , biochemistry , in vitro , receptor , genetics
Acidic fibroblast growth factor (aFGF) stimulated DNA synthesis in primary rat hepatocyte cultures in a dose‐dependent manner with maximal effect at 10–50 ng ml −1 . This activity was dependent on the presence of heparin at a concentration of 10–50 μ·ml −1 . Insulin interacted synergistically with aFGF, as it did with epidermal growth factor (EGF). The response to aFGF was only 50% that found with EGF. The disparity was not due to different kinetics of DNA synthesis, since the peak response for both growth factors occurred at 36–72 hr after plating of the hepatocytes. The potential relevance of this novel hepatocyte mitogen to normal and pathological liver growth is discussed.