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Differentiation of simian virus 40 transformed human mammary epithelial stem cell lines to myoepithelial‐like cells is associated with increased expression of viral large T antigen
Author(s) -
Rudland Philip S.,
Barraclough Roger
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041420328
Subject(s) - myoepithelial cell , antigen , biology , cell culture , immunocytochemistry , microbiology and biotechnology , cell , sv40 large t antigen , virology , immunology , immunohistochemistry , transfection , endocrinology , genetics
Cloned simian virus 40 (SV40)‐transformed human breast epithelial cell lines can differentiate to myoepithelial‐like cells, and these can be isolated as clonal cell lines. Immunofluorescent and immunocytochemical analysis of such cell lines growing on plastic surfaces, collagen gels, and as tumor‐nodules in nude mice indicate that all the cell lines produce SV40 large T antigen, but that the production of this antigen is qualitatively increased in the myoepithelial‐like cells and cell lines. The myoepithelial‐like cell lines produce 4—6 times more immunoprecipitable large T antigen than the parental epithelial cells. The amount of mRNA for large T antigen is also increased by 3.5—5‐fold in the myoepithelial‐like cell lines when analysed by dot‐blot or by Northern hybridisations. Thus, differentiation along the myoepithelial‐like cell pathway is associated in these SV40‐transformed cells with increased expression of the viral large T antigen. It is suggested that immortalization of primary breast epithelial cell cultures may be, in part, due to the expression of large T antigen preventing processes of terminal keratinization.