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Na + /H + antiporter is the primary proton transport system used by osteoclasts during bone resorption
Author(s) -
Hall Tony J.,
Chambers Timothy J.
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041420227
Subject(s) - antiporter , bone resorption , chemistry , resorption , amiloride , osteoclast , proton transport , in vitro , endocrinology , medicine , biochemistry , sodium , membrane , organic chemistry
We have examined the effects of inhibitors of proton transport systems on osteoclastic bone resorption using an in vitro bone slice assay, where osteoclasts (OCs) are free from the influence of other bone cells. Amiloride (AM) and dimethyl‐amiloride (DMA), inhibitors of the Na + /H + antiporter, were potent inhibitors of bone resorption (IC 50 ∼ 9 and 0.7 μM for AM and DMA, respectively). Omeprazole (OM), a potent inhibitor of parietal cell K + /H + ‐ATPase, was a poor inhibitor of OC bone resorption (IC 50 ∼ 100 μM). These results strongly suggest that the Na + /H + antiporter is the primary proton system used by OCs during bone resorption.