Premium
Heat‐induced cytotoxicity in H 2 O 2 ‐resistant Chinese hamster fibroblasts
Author(s) -
Spitz Douglas R.,
Li Gloria C.
Publication year - 1990
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041420206
Subject(s) - chinese hamster , hydrogen peroxide , hamster , cytotoxicity , cell culture , oxidative stress , chinese hamster ovary cell , cell , heat stress , chemistry , hyperthermia , microbiology and biotechnology , biology , in vitro , biochemistry , genetics , zoology , paleontology
Hydrogen‐peroxide‐resistant Chinese hamster fibroblasts, derived from the HA‐1 cell line, were isolated following continuous culturing in the presence of progressively increasing concentrations of hydrogen peroxide. The hydrogen‐peroxide‐resistant phenotype has been stable for over 360 days following removal from H 2 O 2 stress. These H 2 O 2 ‐resisant cell lines demonstrate increased resistance to hyperthermic cell killing, mediated by continuous heating at 43°C but not 45°C. The relationship between mammalian cellular adaptation to oxidative stress mediated by H 2 O 2 and resistance to 43°C hyperthermia is discussed.