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Activation and proliferation signals in murine macrophages: Relationships among c‐fos and c‐myc expression, phosphoinositide hydrolysis, superoxide formation, and DNA synthesis
Author(s) -
Hamilton John A.,
Veis Nurin,
Bordun AnneMarie,
Vairo Gino,
Gonda Thomas J.,
Phillips Wayne A.
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041410321
Subject(s) - dna synthesis , zymosan , superoxide , phospholipase c , microbiology and biotechnology , inositol , chemistry , biology , concanavalin a , biochemistry , dna , signal transduction , in vitro , enzyme , receptor
Murine bone marrow‐derived macrophages (BMM) undergo DNA synthesis in response to growth factors such as colony stimulating factor‐1 (CSF‐1) and granulocyte‐macrophage CSF (GM‐CSF). These macrophages can also be “activated,” but without subsequent DNA synthesis, by a number of other agents, including lipopolysaccharide (LPS), concanavalin A, zymosan, formyl‐methionyl‐leucylphenylalanine (FMLP), and the Ca 2+ ionophore, A23187. When BMM are treated with a range of stimuli, there is some, although not perfect, correlation between transient elevations in both c‐myc mRNA and c‐fos mRNA levels and increases in DNA synthesis. However, enhanced DNA synthesis and oncogene expression are readily dissociated from rises in inositol phosphates and, by implication, phospholipase C‐mediated hydrolysis of phosphatidyl inositol 4,5‐bisphosphate. Superoxide formation in BMM can also be dissociated from the other responses and does not necessarily depend on protein kinase C activation.