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Heat shock stimulates the release of arachidonic acid and the synthesis of prostaglandins and leukotriene B 4 in mammalian cells
Author(s) -
Calderwood Stuart K.,
Bornstein Bruce,
Farnum Elizabeth K.,
Stevenson Mary Ann
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041410214
Subject(s) - arachidonic acid , leukotriene , cyclooxygenase , prostaglandin , phospholipase a2 , lysophosphatidylcholine , heat shock , lipoxygenase , leukotriene b4 , phospholipase , chemistry , prostaglandin e2 , leukotriene c4 , eicosanoid metabolism , eicosanoid , biochemistry , endocrinology , biology , heat shock protein , inflammation , phosphatidylcholine , phospholipid , immunology , membrane , enzyme , asthma , gene
Heat shock has a profound influence on the metabolism and behavior of eukaryotic cells. We have examined the effects of heat shock on the release from cells of arachidonic acid and its bioactive eicosanoid metabolites, the prostaglandins and leukotrienes. Heat shock (42–45°) increased the rate of arachidonic acid release from human, rat, murine, and hamster cells. Arachidonate accumulation appeared to be due, at least partially, to stimulation of a phospholipase A 2 activity by heat shock and was accompanied by the accumulation of lysophosphatidyl‐inositol and lysophosphatidylcholine in membranes. Induction of arachidonate release by heat did not appear to be mediated by an increase in cell Ca + + . Stimulation of arachidonate release by heat shock in hamster fibroblasts was quantitatively similar to the receptor‐mediated effects of β thrombin and bradykinin. The effects of heat shock and β thrombin on arachidonate release were inhibited by glucocorticoids. Increased arachidonate release in heat‐shocked cells was accompanied by the accelerated accumulation of cyclooxygenase products prostaglandin E 2 and prostaglandin F 2α and by 5‐lipoxygenase metabolite leukotriene B 4 . Elevated concentrations of arachidonic acid and metabolites may be involved in the cytotoxic effects of hyperthermia, in homeostatic responses to heat shock, and in vascular and inflammatory reactions to stress.