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Transformed cells overexpress a cytosolic nucleic acid binding protein
Author(s) -
Stoler Daniel L.,
Manly Kenneth F.
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041410117
Subject(s) - nucleic acid , cytosol , biology , dna , microbiology and biotechnology , cell culture , biochemistry , blot , binding protein , virus , dna binding protein , cell , 3t3 cells , transfection , virology , gene , enzyme , genetics , transcription factor
A protein blotting technique was used to identify a 57,000 dalton cytosolic nucleic‐acid‐binding protein found in neoplastically transformed cell lines. Specifically, greater amounts of this protein were found in Kirsten Murine Sarcoma Virus‐, Simian Virus 40‐, and methylcholanthrene‐transformed Balb 3T3 cells than in comparable untransformed cells. An analogous protein was identified in other transformed mammalian cells. Increased levels of the DNA binding protein in sarcoma virus transformants were shown to be dependent on the continued maintenance of the transformed phenotype. The properties of this protein are compared to those of other previously reported nucleic acid binding proteins.