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ATP‐resistant variants of transformed mouse fibroblasts
Author(s) -
Belzer Ilana,
Friedberg Ilan
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041400318
Subject(s) - 3t3 cells , biology , cell culture , cell growth , cell , nucleotide , cytotoxic t cell , membrane permeability , microbiology and biotechnology , biochemistry , membrane , chemistry , in vitro , gene , genetics , transfection
Addition of ATP to cultures of transformed mouse fibroblasts, 3T6 cells, resulted in cell growth inhibition, whereas the growth of the non‐transformed counterparts, 3T3 cells, was only slightly affected. The inhibition was found to be specific for adenine nucleotides, and concentration dependent. At relatively low concentrations (e.g., 0.1 mM) the effect of ATP was cytostatic, whereas at higher concentrations (e.g., 1.0 mM) a cytotoxic effect was exerted. ATP‐resistant variants of 3T6 cells were selected by exposure of cultures to gradually elevated concentrations of ATP. The variants were found to resemble the non‐transformed counterparts, 3T3 cells, more than the 3T6 parent cells, by the following criteria: ATP‐induced alte rations in the membrane potential, changes in membrane permeability, cell growth inhibition, and colony formation on soft agar. The data indicate that long exposure of the transformed cells to external ATP results in redifferentiation and reduction in their tumorigenicity.