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Oxygen gradients in CHO cells: Measurement and characterization by electron spin resonance
Author(s) -
Glockner James F.,
Swartz Harold M.,
Pals Mark A.
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041400315
Subject(s) - oxygen , extracellular , intracellular , electron paramagnetic resonance , limiting oxygen concentration , biophysics , respiration , apparent oxygen utilisation , diffusion , cellular respiration , chemistry , reactive oxygen species , analytical chemistry (journal) , nuclear magnetic resonance , biochemistry , biology , mitochondrion , chromatography , physics , botany , organic chemistry , thermodynamics
The concentration of oxygen within cells is important in many physiological and pathological processes, but such oxygen‐dependent processes are generally studied as a function of the concentration of extracellular oxygen, due to a lack of suitable methods. Using a newly developed technique based on ESR spectroscopy, we show that respiration stimulation of a cell suspension can result in a significant difference between average intracellular and extracellular concentrations of oxygen. These results indicate that studies of oxygen‐dependent phenomena in cells may require measurement of intracellular oxygen concentrations and imply that there are mechanisms in cells that restrict the free diffusion of oxygen.