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TGF‐β stimulates primary human skin fibroblast DNA synthesis via an autocrine production of PDGF‐related peptides
Author(s) -
Soma Yoshinao,
Grotendorst Gary R.
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041400209
Subject(s) - platelet derived growth factor receptor , autocrine signalling , growth factor , dna synthesis , foreskin , transforming growth factor , platelet derived growth factor , biology , fibroblast , microbiology and biotechnology , fibroblast growth factor , dna , cell culture , biochemistry , genetics , receptor
Transforming growth factor‐β (TGF‐β) stimulates DNA synthesis in human foreskin fibroblasts after a prolonged lag period as compared with other growth factors. The mechanism of induction of DNA synthesis appears to be dependent on the synthesis and secretion of PDGF‐related proteins as antibodies which are specific for PDGF can block the TGF‐β‐induced DNA synthesis. Other growth factors such as PDGF, EGF, or FGF do not induce the synthesis of these PDGF‐related proteins. Additionally, TGF‐β treatment of human foreskin fibroblasts induces the expression of the PDGF A‐chain gene but not the B‐chain gene. This phenomenon appears to function in vivo, as subcutaneous injection of TGF‐β in rat skin induces the expression of the PDGF A‐chain gene. These data suggest that TGF‐β may stimulate the growth of fibroblastic cells via an autocrine production of PDGF‐related proteins.