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Protein kinase C modulates effects of prostanoids on cyclic adenosine monophosphate in guinea pig chief cells
Author(s) -
Raufman JeanPierre,
Cosowsky Laurey
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041400112
Subject(s) - adenylyl cyclase , protein kinase c , protein kinase a , forskolin , cholera toxin , activator (genetics) , adenosine , phosphodiesterase , phosphodiesterase 3 , pertussis toxin , phorbol , adcy9 , chemistry , g protein , biology , signal transduction , biochemistry , receptor , endocrinology , kinase , enzyme
In the course of examining the role of protein kinase C in signal transduction in dispersed chief cells from guinea pig stomach, we observed that phorbol esters inhibit prostaglandin (PG)‐stimulated increases in cyclic adenosine monophos‐phate (cAMP). Phorbol 12‐myristate 13‐acetate (PMA), an activator of protein kinase C, decreased maximal levels of PGE 2 ‐stimulated cAMP by 40%. This dose‐dependent effect was observed within 30 sec and was maximal by 1 min of incubation at 37°C. Phorbols that do not activate protein kinase C did not have this effect. Adding H7, a protein kinase C inhibitor, abolished the inhibitory effects of PMA. Adding phosphodiesterase inhibitors did not alter the inhibitory effects of PMA, indicating that these effects are not caused by activation of cyclic nucleotide phosphodiesterases. PMA did not alter the increase in cellular cAMP caused by cholera toxin, forskolin, secretin, or vasoactive intestinal peptide. Hence the site of these prostanoid‐specific actions of protein kinase C does not appear to be stimulatory or inhibitory guanine nucleotide binding proteins or the catalytic component of the adenylyl cyclase system. In dispersed chief cells, activation of protein kinase C may inhibit prostanoid‐induced stimulation of the adenylyl cyclase system by a direct effect on prostaglandin receptors.