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Electrophysiological properties of isolated rat liver cells
Author(s) -
Sawanobori Tohru,
Takanashi Hideo,
Hiraoka Masayasu,
Iida Yoshitaka,
Kamisaka Kazuaki,
Maezawa Hidenori
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041390318
Subject(s) - membrane potential , electrophysiology , depolarization , biophysics , patch clamp , resting potential , voltage clamp , pulse (music) , membrane , chemistry , reversal potential , capacitance , time constant , biology , voltage , biochemistry , electrode , neuroscience , physics , quantum mechanics , electrical engineering , engineering
The electrophysiological properties of isolated rat liver cells were studied using the patch clamp method in whole‐cell configuration. The membrane potential in isolated hepatocytes was −42 ± 17 mV (n = 20). The input resistance (R in ) and the time constant (τ m ) were 51 ± 17 M (the range of 34 to 180 MΩ) (n = 20) and 4.2 ± 1.0 msec (the range of 3 to 16.5 ms) (n = 20). Assuming that the specific membrane capacitance is 1 μF/cm 2 , the membrane resistance and membrane capacitance were 42. ± 9.0 KΩ cm 2 and 87 ± 27 pF. These values indicate that isolated rat hepatocytes are not abnormally permeable or leaky. The current‐voltage relationship was linear with no rectification. The depolarizing pulse from the resting potential did not induce fast or slow inward currents even when norepinephrine or high Ca 2 (3.6 mM) were applied. This indicates that there is no voltage‐sensitive Ca 2+ channel in the isolated hepatocytes.