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Differential effects of follicle‐stimulating hormone, insulin, and insulin‐like growth factor I on hexose uptake and lactate production by rat Sertoli cells
Author(s) -
Oonk Ria B.,
Jansen Ruud,
Grootegoed J. Anton
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041390128
Subject(s) - medicine , endocrinology , insulin , sertoli cell , glucose uptake , biology , extracellular , hexose , hormone , follicle stimulating hormone , chemistry , spermatogenesis , biochemistry , luteinizing hormone , enzyme
The stimulatory effects of follicle‐stimulating hormone (FSH), insulin, and insulin‐like growth factor I (IGF‐I) on lactate production and hexose uptake by Sertoli cells from immature rats were studied. The time‐courses and the maximal stimulatory effects of FSH, insulin, and IGF‐I on lactate production were virtually identical. When Sertoli cells were incubated in the presence of FSH in combination with insulin or IGF‐I (submaximal doses), additive but no pronounced synergistic effects were observed. The stimulatory effects of FSH and insulin were not dependent on the presence of extracellular calcium. 2‐Deoxy‐D‐glucose (2‐DOG), an analogue of D‐glucose, was used to investigate the hexose transport system of Sertoli cells. Uptake of 2‐DOG was linear in time and virtually all of the intracellular 2‐DOG was phosphorylated up to 30 min of incubation; 2‐DOG uptake was inhibited by c ytochalasin B, but not by cytochalasin E. D‐glucose, but not D‐galactose, appeared to be an effective competitor of 2‐DOG uptake. The K m of 2‐DOG uptake was not influenced by FSH, insulin, and IGF‐I. FSH had no effect on the V max of 2‐DOG uptake, whereas insulin and IGF‐I caused a 30% stimulation of the V max . It is concluded that FSH, insulin, and IGF‐I stimulate lactate production by cultured Sertoli cells, but that only insulin and IGF‐I stimulate hexose transport. The insulin‐like effect of FSH or Sertoli cells may principally involve stimulation of glycolytic enzyme activities.