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Growth of normal human keratinocytes and fibroblasts in serum‐free medium is stimulated by acidic and basic fibroblast growth factor
Author(s) -
Shipley Gary D.,
Keeble Winifred W.,
Hendrickson Jill E.,
Coffey Robert J.,
Pittelkow Mark R.
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041380310
Subject(s) - basic fibroblast growth factor , fibroblast , keratinocyte , fibroblast growth factor , epidermal growth factor , cell growth , in vitro , growth factor , biology , cell culture , cell division , microbiology and biotechnology , foreskin , endocrinology , fibroblast growth factor receptor 3 , medicine , cell , biochemistry , receptor , genetics
Keratinocytes and fibroblasts isolated from human neonatal foreskin can be plated and grown through multiple rounds of division in vitro under defined serum‐free conditions. We utilized these growth conditions to examine the mitogenic potential of acidic and basic fibroblast growth factor (aFGF and bFGF) on these cells. Our results demonstrate that both aFGF and bFGF can stimulate the proliferation of keratinocytes and fibroblasts. aFGF is a more potent mitogen than bFGF for keratinocytes. In contrast, bFGF appears to be more potent than aFGF in stimulating the growth of fibroblast cultures. Heparin sulfate (10 μg/ml) dramatically inhibited the ability of bFGF to stimulate the proliferation of keratinocytes. In comparison, heparin slightly inhibited the stimulatory effect of aFGF and had no effect on epidermal growth factor (EGF) stimulation in keratinocyte cultures. In fibroblast cultures the addition of heparin enhanced the mitogenic effect of aFGF, had a minimal stimulatory effect on the mitogenic activity of bFGF, and had no effect on EGF‐stimulated growth. Our results demonstrate that the proliferation in vitro of two normal cell types found in the skin can be influenced by aFGF and bFGF and demonstrate cell‐type specific differences in the responsiveness of fibroblasts and keratinocytes to these growth factors and heparin.