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Enhancement of plasminogen activator activity in cultured endothelial cells by granulocyte colony‐stimulating factor
Author(s) -
Kojima Soichi,
Tadenuma Hirohiko,
Inada Yuji,
Saito Yuji
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041380125
Subject(s) - extracellular , plasminogen activator , fibrin , tissue plasminogen activator , endothelial stem cell , activator (genetics) , cell culture , granulocyte colony stimulating factor , intracellular , colony stimulating factor , endothelium , granulocyte , chemistry , biology , microbiology and biotechnology , medicine , endocrinology , immunology , in vitro , biochemistry , haematopoiesis , stem cell , chemotherapy , genetics , gene
A hitherto unknown function of granulocyte colony‐stimulating factor (G‐CSF) was found using cultured endothelial cells. G‐CSF stimulated activity of plasminogen activator (PA) in both extracellular and intracellular milieus of endothelial cells obtained from bovine carotid and aortic artery. This effect was dependent on the concentration of G‐CSF added to the culture medium and on the treatment time. The extracellular activity was enhanced approximately 5‐fold at a concentration of 5,000 colony‐forming unit (CFU)/ml (2.6 nM) and in about a 15‐hr treatment period. Analyses by fibrin and reverse fibrin autography revealed that activity of PA was much more increased than that of PA inhibitor in endothelial cells treated with G‐CSF.