Premium
Altered formation of DNA replication intermediates in cells growing in different culture conditions
Author(s) -
lönn Ulf,
Lönn Sigrid,
Nylen Urban,
Winblad Gerard
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041380108
Subject(s) - replicon , dna , dna replication , dna synthesis , biology , cell culture , microbiology and biotechnology , replication (statistics) , chemistry , biochemistry , genetics , virology , plasmid
In human melanoma cells one can detect two discrete DNA replication intermediates, 10‐kb DNA intermediates and Okakzaki fragments. Both intermediates are seen when cells are rapidly growing in medium supplemented with fetal calf serum. When the medium is supplemented with newborn calf serum, one can detect Okakzaki fragments but not 10‐kb DNA intermediates. In contrast we do not detect changes in the replicon sizes in the two media.