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Stochastic model for mast cell proliferation in culture of murine peritoneal cells
Author(s) -
Kobayashi Toshimi,
Nakahata Tatsutoshi
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041380105
Subject(s) - mast cell , safranin , percoll , biology , transdifferentiation , microbiology and biotechnology , cell type , cell , immunology , in vitro , biochemistry , staining , genetics
We recently identified two types of mast cell colonies derived from murine peritoneal cells: type 1 and type 2. Type 1 mast cell colonies consisted of berberine sulfate(+)‐ safranin(+) connective tissue‐type mast cells (CTMC) and were derived from mature CTMC in the heaviest fraction obtained by Percoll density gradient centrifugation. In contrast, type 2 mast cell colonies consisted of alcian blue(+)‐ berberine sulfate(−)‐ safranin(−) mucosal mast cells (MMC) and were derived from immature progenitors in low density fractions. We replated a total of 60 type 1 and 60 type 2 mast cell colonies and examined their capability for producing secondary colonies. Although all of the primary colonies yielded secondary colonies, the replating efficiencies of individual colonies varied over a wide range. Cumulative distributions of secondary colonies from both type 1 and type 2 primary colonies could be fitted well by gamma distributions obtained by computer simulation. These findings are in agreement with the stochastic model for CTMC‐ and MMC proliferation. Cytological analyses of secondary colonies from primary type 1 colonies revealed heterogeneous distributions of alcian blue(+)‐ safranin(−)‐ berberine sulfate(−) mast cells, suggesting that transdifferentiation from mature CTMC to safranin(−)‐ berberine sulfate(−) mast cells is also governed by stochastic mechanisms.