Premium
L‐ascorbic acid 2‐phosphate stimulates collagen accumulation, cell proliferation, and formation of a three‐dimensional tissuelike substance by skin fibroblasts
Author(s) -
Hata RyuIchiro,
Senoo Haruki
Publication year - 1989
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041380103
Subject(s) - ascorbic acid , extracellular matrix , glycosaminoglycan , chemistry , fibroblast , procollagen peptidase , endoplasmic reticulum , cell growth , biochemistry , cell culture , extracellular , microbiology and biotechnology , biology , in vitro , genetics , food science
Proliferation of human skin fibroblasts was stimulated significantly by the presence of L‐ascorbic acid 2‐phosphate (Asc 2‐P). The presence of Asc 2‐P (0.1–1.0 mM) in the culture medium for 3 weeks enhanced the relative rate of collagen synthesis to total protein synthesis 2‐fold as well as cell growth 4‐fold. Coexistence of L‐azetidine 2‐carboxylic acid (AzC), an inhibitor of collagen synthesis, attenuated both effects of Asc 2‐P in a dose‐dependent manner. Supplementation of the medium with Asc 2‐P also accelerated procollagen processing to collagen and deposition of collagen in the cell layer. Among the acidic glycosaminoglycans (GAG), another major component of extracellular matrix (ECM), deposition of sulfated forms was increased by the additive. Electron microscopic observations showed multilayered, rough endoplasmic reticulum‐rich cells surrounded by dense ECM. These results indicate that Asc 2‐P is useful in culture systems as a long‐acting vitamin C derivative and also that it promotes reorganization of a three‐dimensional tissuelike substance from skin fibroblasts in culture by stimulating collagen accumulation in the fibroblasts.