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Role of plasma membrane and of cytomatrix in maintenance of intracellular to extracellular ion gradients in chicken erythrocytes
Author(s) -
Cameron I. L.,
Hunter K. E.,
Smith N. K. R.,
Hazlewood C. F.,
Ludany A.,
Kellermayer M.
Publication year - 1988
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041370213
Subject(s) - extracellular , cytoplasm , intracellular , membrane , cytoskeleton , ultrastructure , cell membrane , microbiology and biotechnology , biophysics , cell , biochemistry , biology , chemistry , anatomy
Abstract Ultrastructural observations in combination with electron probe X‐ray microanalysis on detergent (Brij 58) permeabilized (disruption of the plasma membrane) nucleated chicken erythrocytes support the view that a large fraction of cytoplasmic and nuclear K + is not freely diffusible and that adsorption of K + on detergent released mobilizable proteins exists within the cell. The data also suggest that the detergent proteins are normally immobilized by a detergent‐resistant cytoskeleton so that they are not immediately free to diffuse from the cell for several minutes after detergent disruption of the plasma membrane.